This dataset includes 70 sets of 3D confocal high-resolution images. All images were imaged using an LSM800 Zeiss microscope with a Plan-apochromat 1.40-NA, 63× objective, and Zeiss ZEN Blue 2.6 software was used to acquire the images. Three channels were used to acquire transmitted light (TL), SYBR GoldTM- (Thermo Fisher Scientific, Inc.) labeled (nuclear and mitochondrial DNA), and TMRM-labeled (mitochondria) images. Each confocal image consists of 32 slices with an interval of 0.15 µm and a YX resolution of 917 × 917 pixels.

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Dataset of fluorescent mice brain vessels Confocal 3D volumes aligned to Light-Field images.

Confocal:

  • Single volume dimension: 1287x1287x64.
  • Number of samples: 362
  • Voxel size: 0.086x0.086x0.9 um.
  • Objective: 40x/1.3 Oil.
  • Stain: tomato lectin (DyLight594 conjugated, DL-1177, Vector Laboratories).

 

LightField:

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117 Views

The dataset contains high-resolution microscopy images and confocal spectra of semiconducting single-wall carbon nanotubes. Carbon nanotubes allow down-scaling of electronic components to the nano-scale. There is initial evidence from Monte Carlo simulations that microscopy images with high digital resolution show energy information in the Bessel wave pattern that is visible in these images. In this dataset, images from Silicon and InGaAs cameras, as well as spectra, give valuable insights into the spectroscopic properties of these single-photon emitters.

Instructions: 

The dataset is generated with docker containers from the measurement data. The measured data is in Igor Binary Waves. The specific format can be read with a custom reader an processed with various tools.

Processing will be applied automatically to various output formats using docker containers.

 

See current development status and dataset description will be updated on

https://gitlab.com/ukos-git/nanotubes

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455 Views

Datasets of confocal microscopy images of cardiomyocytes aimed at development of image recognition systems. Images of live and healthy cardiomyocytes with fluorescently stained sarcolemma were assigned into 5 classes according to their development stages. The least developed cardiomyocytes were considered to be at stage 1 (class 1) while the most developed ones were assigned stage 5 (class 5). All other images belong to a class 0.

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274 Views