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Diabetic Retinopathy is the second largest cause of blindness in diabetic patients. Early diagnosis or screening can prevent the visual loss. Nowadays , several computer aided algorithms have been developed to detect the early signs of Diabetic Retinopathy ie., Microaneurysms. The AGAR300 dataset presented here facilitate the researchers for benchmarking MA detection algorithms using digital fundus images. Currently, we have released the first set of database which consists of 28 color fundus images, shows the signs of Microaneurysm.