This dataset protocol details the acquisition of a surface electromyography (sEMG) dataset from the Tibialis Anterior (TA) and Gastrocnemius Lateralis (GL) muscles of 20 healthy adults, with an equal distribution of 10 male and 10 female participants. The data was collected using the Delsys Trigno wireless EMG system during a 30-second walking session. Proper electrode placement on the specified muscles was ensured for accurate signal capture. Ethical considerations were addressed, with approval from the Institutional Review Board and informed consent from participants.