Anatomical landmark trajectories are commonly used to define joint coordinate systems in human kinematic analysis according to standards proposed by the International Society of Biomechanics (ISB). However, most inertial motion capture (IMC) studies focus only on joint angle measurement, which limits its application. Therefore, this paper proposes a new method to calculate the trajectories of anatomical landmarks based on IMC data. The accuracy and reliability of this method were investigated by comparative analysis based on measurement data from 16 volunteers.