The I Scan 2 scanner from Cross-Match Technologies was used to acquire all data. Iris images are captured in near-infrared wavelength band (700-900 nm) of the electromagnetic spectrum. All images were acquired in SAP laboratory of computer science and engineering department of Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. The subject images were acquired during the span of 7 to 8 months in years 2017 and 2018.  GMBAMU-IRIS dataset contains total 5616 images from 312 subjects.