Dataset for testing the performance of colorful FPM reconstruction

Citation Author(s):
Jizhou Zhang
Submitted by:
Jizhou Zhang
Last updated:
Tue, 05/17/2022 - 22:17
DOI:
10.21227/H2QQ2V
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Abstract 

This dataset is a human blood smear section FPM dataset for testing the performance of colorful FPM reconstruction. The subdirectories 'R', 'G'  and 'B ' contain images corresponding to red, green and blue light with 30ms exposure time. The subdirectory 'W' contains the multiplex images with 10ms exposure time. The subdirectory 'K' contains one dark-frame for using dark-frame denoising method. The subdirectory '20X' contains high-resolution images captured with a 20X objective lens for comparison.

Instructions: 

% This dataset is a human blood smear section FPM dataset for testing the 

% performance of colorful FPM reconstruction. The subdirectories 'R', 'G' 

% and 'B ' contain images corresponding to red, green and blue light with 

% 30ms exposure time. The subdirectory 'W' contains the multiplex images 

% with 10ms exposure time. The subdirectory 'K' contains one dark-frame for 

% using dark-frame denoising method. The subdirectory '20X' contains 

% high-resolution images captured with a 20X objective lens for comparison.

% The parameters of our system are as follows.

% 2018/05/02, Jizhou Zhang.

 

%---------------------ALL DIMENSIONS ARE IN MICRONS------------------------

%---------------------parameters of the microscope------------------------

image_px = 6.5;                 % the actual pixel size of sensor

magnification = 4;              % the magnification of the objective

uniform_px = image_px/magnification;% the uniform pixel size

wavelength_R = 0.629;             % wavelength of light used for simulated illumination

wavelength_G = 0.505;             % wavelength of light used for simulated illumination

wavelength_B = 0.460;             % wavelength of light used for simulated illumination

LED_spacing = 8128;             % distance between LEDs in the array

illumination_distance = 98000;% distance from the LED matrix to the object

NA_obj = 0.13;                  % Numerical aperture of simulated imaging system

%---------------------parameters of the LED matrix------------------------

illumination_layers = 7;        % no of layers of a spiral square matrix of LEDs used to illuminate the setup

N = 2*illumination_layers - 1;% side of square of illumination matrix

dx_R = 0;       dy_R = 0;       % the position deviation of red led

dx_G = -460;dy_G = 0;       % the position deviation of green led

dx_B = 770;     dy_B = 0;       % the position deviation of blue led

LED_x = double( ((1:N)-illumination_layers)*LED_spacing );    % the x position of LEDs

LED_y = double( ((1:N)-illumination_layers)*LED_spacing );    % the y position of LEDs

[LED_x, LED_y] = meshgrid(LED_x, LED_y);            % the coordinate matrix

LED_num = N*N;                  % number of LED

LED_x = reshape(LED_x',[1,LED_num]);

LED_y = reshape(LED_y',[1,LED_num]);

LED_R_x = LED_x - dx_R;

LED_R_y = LED_y - dy_R;

LED_G_x = LED_x - dx_G;

LED_G_y = LED_y - dy_G;

LED_B_x = LED_x - dx_B;

LED_B_y = LED_y - dy_B;

%---------------------parameters of the image patch------------------------

cx = 1280;                      % the x position of image center

cy = 1080;                      % the y position of image center

patch_cx = 1300;                % the x position of image patch

patch_cy = 1020;                % the x position of image patch

half_width = 80;                % the half width of image patch

 

 

Dataset Files

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