The Web is essential for education and e-learning. This situation has been boosted by migration to distance education due to the SARS-CoV-2. However, students with disabilities have been seriously affected because online teaching is very often not accessible. For this reason, this research aims to evaluate the accessibility of the home pages of the web portals of the Ecuadorian higher education institutions ranked in the Webometrics with the Web Content Accessibility Guidelines (WCAG) 2.1 of the World Wide Web Consortium.