Rhodamine concentration with different backgrounds

Citation Author(s):
Submitted by:
Ambar Perez-Garcia
Last updated:
Thu, 06/13/2024 - 15:18
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This dataset in conformed by hyperspectral images captured with the Fx10 camera. The images show the dissolutions of different concentrations of rhodamine. The Rhodamine Water Tracer, hereafter referred to as rhodamine, is a fluorescent dye primarily used as a tracer in aquatic environments. The rhodamine was purchased from ThermoFisher Scientific, identified by the chemical codes CAS 37299-86-8 and 7732-18-5, with catalogue number 446971000. Initially, the rhodamine is highly concentrated at 20%, or 200 g/L, requiring dilution in water to achieve an appropriate concentration for discharge into the sea. 

This study utilizes 250 mL solutions of seawater and distilled water with varying concentrations of rhodamine. Dissolutions with different concentrations of rhodamine are produced: 1 mg/L, 10 mg/L, 15 mg/L, and 30 mg/L. In addition to the rhodamine dilutions, pure seawater and distilled water samples are also included in the study. These pure samples serve as reference spectra and are essential for the comparative analysis of rhodamine-contaminated samples. The same images are taken with two backgrounds, una light and another dark. The data can be used to test machine learning algorithms such as classification methods.


The spectral images of the Fx10 are organized into different folders:

  • Distilled-dark
  • Distilled-light
  • Pure-samples-dark
  • Pure-samples-light
  • Seawater-dark
  • Seawater-light

The names are quite representative, with light and dark indicating the type of background they were taken against, and distilled and seawater indicating the type of water. The pure samples folders contain exclusively distilled and pure seawater samples, without any rhodamine.

Each folder contains several files:

  • Black reference (BR.bin and BR.hdr)
  • White reference (WR.bin and WR.hdr)
  • Raw image (output.bin and output.hdr)
  • Calibrated image (calibratedImage.hdr, calibratedImage.img, calibratedImage.enp, calibratedImage.png)
  • Metadata (info.json)

Data can be used for spectral analysis or classification studies in tools such as ENVI or in a programming environment such as Python.