This dataset in conformed by hyperspectral images captured with the Fx10 camera. The images show the dissolutions of different concentrations of rhodamine. The Rhodamine Water Tracer, hereafter referred to as rhodamine, is a fluorescent dye primarily used as a tracer in aquatic environments. The rhodamine was purchased from ThermoFisher Scientific, identified by the chemical codes CAS 37299-86-8 and 7732-18-5, with catalogue number 446971000.