Biomolecular Adsorption Database (BAD)

The Biomolecular Adsorption Database, BAD, is an archive of the data regarding protein adsorption on flat solid surfaces, as reported in peer-reviewed literature. BAD comprises only data that quantitatively report, completely, the descriptors of all three classes of descriptors relevant to protein adsorption: (i) the adsorbed protein, preferably identifiable in the Protein Data Bank (PDB); to allow further derivation of protein descriptors; (ii) the flat surface (type, water contact angle or surface tension); and (iii) fluid descriptors, i.e., protein concentration in solution, pH, ionic strength, and temperature. BAD2.0 also comprises auxiliary data, i.e., method of measurement, and the DOI of the relevant references for easy retrieval. The source data of BAD 2.0 comprise experimental results from adsorption isotherms, plateaus of adsorption kinetics experiments, and single adsorption experiments (when surfaces are incubated in a protein olution with a known initial protein concentration). The primary data was collected from the open literature using the main literature search engines, e.g., Scopus, Google Scholar, and ISI Web of Science, and using several combinations of relevant keywords, e.g., (protein adsorption)-AND((contact angle)OR(surface tension)). The initial collection of data was followed by critical analysis of the results and augmentation of the published data, e.g., contact angles for identical surfaces, whenever possible. BAD comprises 865 protein adsorption records. In several instances, the pair of values of (protein concentration in solution) vs (protein concentration on the surface) had to be estimated from the original contributions through graphical interpolation of data points in the original figures. 78.5% of the data collected in BAD are the result of adsorption isotherms data, and 21.5% from single adsorption experiments. Only adsorption data from solutions containing a single protein are reported in BAD. While the focus of the data is presented in the core BAD table, there are additional tables describing the BAD protein properties and the output values obtained by a molecular surface calculator (ProMS - Protein Molecular Surface Calculator).