A novel image deblurring dataset for materials science and light-optical microscopy. This dataset provides images with real out-of-focus and motion blur and a sharp reference image for each observation. The dataset includes image samples of lithium-ion batteries, Fe-Nd-B sintered magnets, 100Cr6 steel with a partially bainitic microstructure, and aluminium-silicon casting alloys. The dataset was acquired using a ZEISS AxioImager.Z2 Vario light microscope and the 6-megapixel camera Axiocam 506 color.