3 different cell-based assays were conducted to characterize the functional activity of samples, providing data regarding the effector function of antibodies induced by RV144 including: gp120-specific antibody dependent cellular phagocytosis (ADCP) by monocytes, antibody dependent cellular cytotoxicity (ADCC) by primary NK cells, and NK cell cytokine release (namely the combination of IFNγ, MIP-1β, and CD107a).