RV144 Vaccine

Citation Author(s):
US Military HIV Research Program
US Military HIV Research Program
Submitted by:
FERDI SARAC
Last updated:
Wed, 05/04/2022 - 07:33
DOI:
10.21227/xsvv-e897
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Abstract 

3 different cell-based assays were conducted to characterize the functional activity of samples, providing data regarding the effector function of antibodies induced by RV144 including: gp120-specific antibody dependent cellular phagocytosis (ADCP) by monocytes, antibody dependent cellular cytotoxicity (ADCC) by primary NK cells, and NK cell cytokine release (namely the combination of IFNγ, MIP-1β, and CD107a).

Instructions: 

3 different cell-based assays were conducted to characterize the functional activity of samples, providing data regarding the effector function of antibodies induced by RV144 including: gp120-specific antibody dependent cellular phagocytosis (ADCP) by monocytes, antibody dependent cellular cytotoxicity (ADCC) by primary NK cells, and NK cell cytokine release (namely the combination of IFNγ, MIP-1β, and CD107a). Antibody features were assessed using a customized microsphere array to characterize the antibodies induced by the vaccine in terms of their antigen specificity (gp140, gp120, V1V2, gp41, and p24) and IgG subclass (IgG1, IgG2, IgG3, and IgG4). For both the array-generated antibody feature data, and cell-based assay assessment of antibody functional activity, excellent discrimination between placebo (n = 20) and vaccinated (n = 80) subjects was observed (Choi et al. 2015). Further information can be gathered from US Military HIV Research Program and a previous study: https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1....