Datasets
Standard Dataset
Small intestinal serosa voltage dataset during ex vivo blood reperfusion and analysis script
- Citation Author(s):
-
SatoshiMuto
Tokyo Metropolitan University - Submitted by:
- Satoshi Muto
- Last updated:
- Tue, 03/04/2025 - 02:51
- DOI:
- 10.21227/144e-wg94
- Data Format:
- License:
25 Views- Categories:
- Keywords:
Abstract
Small intestinal serosa voltage is defined as a potential in the serosa relative to the mesentery, which provides good visualization of the neural activity and the health of the electrical activity of the Interstitial Cells of Cajal that control contractions. This dataset consists of a .csv format of porcine small intestinal serosa voltage measured during ex vivo blood reperfusion, which mimics the damage to the organ during transplantation. The MATLAB code performs the digital filtering, the wavelet analysis, the autocorrelation function calculation, and the peak frequency analysis and outputs as a single figure. This content could be applied to construct a technique evaluating the function of the small intestine before transplantation. This method has the potential to be utilized not only to regulate the contraction of the intestinal organoid but also to provide insights that could inform the treatment of associated diseases.
Using the Dataset
- Download the dataset
- Extract the dataset.
- Once you open the .csv file, you will see two columns of numbers: column A is the measurement time (ms), and column B is the raw data of the measured small intestinal serosa voltage (-).
Brief Description of the File Name and Experimental Conditions of the Dataset
- Cold Storage: Transplanted organs are immersed in an organ preservation solution maintained at a low temperature.
- Hypothermic Machine Perfusion: Transplanted organs are continuously perfused with an organ preservation solution at a low temperature. In this study, the position-dependence of the intestine was investigated. The data measured at approximately the center of the intestine are saved in "At_the_center_of_the_small_intestine." The data measured at the edge are stored in "At_the_edge_of_the_small_intestine."
- Warm Ischemia: The blood supply is cut off at body temperature. The organ is intentionally damaged.
- Blood Reperfusion: An operation in which blood is pumped continuously to simulate some of the disorders that occur during transplantation.
*The operations were performed in the order indicated in the filenames, and the voltage measurements were performed during blood reperfusion.
Analysis of the Dataset
- Open MATLAB (R2023a and R2024b) and install the add-ons ("Wavelet Toolbox" and "Signal Processing Toolbox") to run the scripts.
- Open SigProc.m in the same location as the .csv format you want to analyze.
- Without changing any parameters, press Run to execute the program.
- The analysis results will be displayed. The time waveform and the scalogram of small intestinal serosa voltage filtered by frequency band are shown on the left side of the figure. The autocorrelation function and the peak frequency are shown on the right side. The measurement time of the data and the time length used for the analysis are displayed in the Command Window. In addition, the figure displayed on the display is saved in .png format.
