This dataset comprises high-resolution imaging data of biological porcine, clinically approved porcine and bovine, and chick embryo heart tissues. The dataset includes comprehensive anatomical and structural details, making it valuable for research in cardiovascular biology, tissue engineering, and computational modeling. The porcine and bovine heart samples are clinically approved, ensuring relevance for translational and preclinical studies. The chick embryo heart data provides insights into early cardiac development.