Drought has become one of the main challenges facing global agricultural production and crop safety. Drought stress will lead to the termination of crop photosynthesis and metabolic disorders, which will seriously affect the growth and development of crops. We aimed to study a method for identificaton of the drought stress in tomato seedlings using chlorophyll fluorescence imaging. In this study, chlorophyll fluorescence parameters and there corresponding chlorophyll fluorescence images of 4 different drought stress levels were collected.