The popularity of wearable physiological recording devices has opened up new possibilities for the assessment of personality traits in everyday life. Compared with traditional questionnaires or laboratory assessments, wearable device-based measurements can collect rich data about individual physiological activities in real-life situations without interfering with normal life, enabling a more comprehensive description of individual differences. The present study aimed to explore the assessment of individuals’ Big-Five personality traits by physiological signals in daily life situations.