Objective: To analyze the effect of electrical pulses (EP) with tomato lipophilic extract (TLE) on human, triple negative breast cancer (TNBC) MDA-MB-231 cells and non-cancerous breast MCF-10A cells and to gain insights into the mechanisms of action. Methods: We prepared TLE using solvent extraction method. We identified the constituents of TLE using LC-MS analysis. TLE with the dose of 250μg/ml was applied with eight, 800V/cm, 100μs pulses to MDA-MB-231 and MCF-10A cells and their viability was studied.