The proposed SDTB dataset is collected from microscopic testicular tissue sections of 15 patients diagnosed with azoospermia. It simulates the process of selecting high-quality sperm in a testicular puncture scenario for further infertility diagnosis and treatment. Specifically, a testicular puncture is performed on these patients, followed by cleansing and shredding of the tubule tissue. The samples are then examined under a Nikon ECLIPSS Ti microscope at 200× magnification.