We construct the Thyroid Nodule Ultrasound (TNUS) dataset with thyroid nodule positions and puncture annotations, lacking in existing datasets. It supports future research in automating detection and diagnosis, enhancing diagnostic accuracy and clinical applications. The TNUS dataset is a curated collection of thyroid nodule ultrasound (US) images designed to support research in puncture position detection and nodule segmentation. It contains 4,376 images with puncture position annotations and 2,626 additional images with thyroid/nodule masks.