Ultrasound (US) provides non-invasive visualization of tissue morphology for musculoskeletal disorders. Spatial Frequency Analysis (SFA) of US images quantitatively characterizes tissue morphology, and has shown the ability to distinguish healthy from pathological tendons. However, the impact of US machine settings on SFA for tendon pathology remains underexplored. Methods: Five participants with unilateral supraspinatus tendon partial tears were imaged bilaterally to examine how variations in US settings (frequency, dynamic range, gain) influence SFA parameters.